April 29, 2015 – 1:00 pm (EDT): Understanding Camera Technology for Light Sheet Microscopy with Keith Bennett, Ph.D., Consultant
No scientific camera is perfect; there are always tradeoffs between noise, field of view, resolution, speed and throughput, full well capacity, dynamic range, quantum efficiency and pixel uniformity. New functionality for controlling and synchronizing the camera shutter with illumination and excitation in scientific CMOS cameras (sCMOS), such as light-sheet modeTM, and global exposure timing bring previously unavailable capabilities to researchers. Bennett will describe the architectures of CCD, EMCCD, and sCMOS camera technologies. He will emphasize the relevance of these differences for life science research, focusing on application to light sheet microscopy.
Feb. 25, 2015 – 1:00 pm (EST): Using Dynamic Intravital and Advanced Multiplex Static Ex Vivo Imaging to Visualize Immune System Function with Ron Germain, National Institute for Allergy and Infectious Diseases, NIH
Germain covered the application of intravital 2-photon microscopy and highly multiplexed ex vivo confocal imaging to the analysis of immune function in complex tissues, including intravital imaging experiments, major findings using the method, and future developments.
Oct 17, 2014 – 4:00 pm (EDT): Bringing Bioelectricity to Light with Adam Cohen, Harvard University
The combination of optical perturbation and optical readout of membrane voltage opens the door to studies of electrophysiology in a huge variety of systems previously inaccessible to electrode-based measurements. Yet the application of optogenetic electrophysiology requires careful reconsideration of the fundamentals of bioelectricity. Cohen will cover fundamental aspects of bioelectricity and new tools for all-optical electrophysiology.
Sept 17, 2014 – 2:00 pm (EDT): Imaging Life at High Spatiotemporal Resolution with Eric Betzig, HHMI Janelia Farm Research Campus
The imaging of living systems involves inevitable tradeoffs between resolution, speed, non-invasiveness, and imaging depth. Betzig will describe new technologies that balance these tradeoffs and allow us to image molecules, living cells, or embryos either at nanometric resolution in 2D, at hundreds of planes/sec for hundreds of time points in 3D, or with optimal diffraction-limited resolution across whole organisms.
July 16, 2014 – 2:00 pm (EDT): Navigating the Cellular Landscape through Imaging with Jennifer Lippincott-Schwartz, National Institute of Child Health and Human Development, NIH
Emerging visualization technologies are capturing processes at the level of whole organisms down to single molecules. Lippincott-Schwartz will discuss new fluorescence imaging techniques and the ways they are helping researchers navigate through the cell to unravel long-standing biological questions.