From Microscope to Figure

Doing cell biology experiments can be tough. Weeks of pilot work, failed attempts, and finally one day, getting a nice result. Having done the hard work, how do you make your data shine?

In this video, focusing on microscopy experiments, we will cover how to take great images, analyze them and get a nice looking figure at the end. The emphasis will be on reproducibility, removing bias from analysis, and underscoring current best practice in cell biology.

Webinar Resources:

• The Digital Cell: Cell Biology as a Data Science
• Full-length tutorial for making figure panels
• Cynthia Brewer’s color tables
• Paul Tol’s stylesheet
• Paletton (designing colour palettes)
• Fiji
• RStudio
• Inkscape
• SuperplotsOfData
• Webinar Q&A responses

Moderator:

Lorena Benedetti, Postdoctoral Fellow, Jennifer Lippincott-Schwartz

Member of ASCB’s Public Information Committee

Speaker:

Steven Royle, Professor, University of Warwick

Stephen J. Royle is a Professor in the Centre for Mechanochemical Cell Biology, Warwick Medical School at the University of Warwick, UK. He runs a research group working on cell division and membrane traffic in human cells. Steve’s lab pursue a quantitative approach to cell biology and he has written a handbook “The Digital Cell” published by Cold Spring Harbor Laboratory Press on using computers in cell biological research. He maintains a web resource (quantixed.org) that focusses on data visualization and coding. Steve was awarded the 2021 Hooke Medal for Cell Biology from the British Society for Cell Biology. He can be found on Twitter as @clathrin.